Algorithms predict siRNAs against conserved regions within viruses. Nanoparticles can deliver the siRNAs to the targeted tissue. Using siRNAs delivered through intranasal inhalation we have demonstrated rescue of animals from exposure to a 10x LD50 of H5N1 (avian flu) – a dose that will kill all animals within 10 days. The siRNAs are able to specifically result in silencing of the viral gene and limits its replication.  By targeting more than one viral gene in the same cocktail we significantly reduce the ability for the virus to escape therapeutic pressure through gene mutation.

 

 

siRNA sequences are gene-specific and these and the nanoparticles can be rapidly synthesized in large quantities to act as an interim measure during the production and scale-up of vaccines.

Furthermore, SiRNAs delivered via nasal spray have shown prophylactic as well as therapeutic effects and could be beneficial to both young and elderly patients who do not respond well to vaccines.

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